Biodiesel is one of notable alternatives to widely used petroleum-derived diesel fuel since it can be generated by domestic natural resources such as palm oil and vegetables oils. Cooking oil is one of palm oil that has been used for production of biodiesel oil. Previous study reported the production of biodiesel from low grade cooking oil using homogeneous catalyst and it give several drawbacks. Therefore, in this study the characterization of cooking oil and biodiesel oil have been done using heterogenous catalysts, which is calcium oxide-based catalyst supported on alumina. The transesterification of low-grade cooking oil is carried out for 2 hours, at 60-65 C reaction temperature; and the methanol:oil ratio 18:1. The physico-chemical properties of biodiesel were tested to evaluate the viscosity, flash point, cloud point, pour point, acid value and saponification value of biodiesel using American Society for Testing and Materials (ASTM) method. The presence of functional group in biodiesel oil was observed using FTIR analysis. The presence of functional group in biodiesel oil was observed using FTIR analysis. The peak at 3325.63 cm-1 shows the OH- bonding in biodiesel oil. Meanwhile, the region between 1700 cm-1 and 1800 cm-1 presents a strong absorption band corresponding to stretching vibrations of the carbonyl (C=O) functional group; which is esters from the low-grade cooking oil and biodiesel with absorbance at 1743.72 cm-1 and 1743.94 cm-1 respectively.