HANISAH Izati Adli1^a, AN’AMT Mohamed Noor1^b*, NOR HAKIMIN Abdullah1^c, ARLINA Ali1^d and NORFADHILAH Ibrahim1^e  

¹Advanced Materials Research Cluster, Faculty of Bioengineering and Technology, Universiti Malaysia Kelantan, Jeli Campus, Jeli, Kelantan 17600, Malaysia

^aizati.a@umk.edu.my, ^banamt@umk.edu.my, ^cnorhakimin@umk.edu.my, ^darlina@umk.edu.my ^enfadhilah@umk.edu.my

Abstract. The effect of the silver complex on graphene oxide and its antibacterial studies was reported in this work. Graphene oxide (GO) has a high specific surface area and is well-known for being an ideal substrate material for growing silver nanoparticles. The simplified Hummer's method was chosen to prepare GO and Silver nanoparticles (AgNPs), which have been decorated on the surface of GO during the reduction of silver complex solution [Ag(NH3)2OH] in GO aqueous solution. The obtained nanocomposite was analyzed by using Ultraviolet-visible spectrophotometry (UV-Vis) and X-ray diffraction (XRD). The antibacterial performance against Gram-negative bacteria (Escherichia coli) and Gram-positive bacteria (B. subtilis) was investigated using the disk agar diffusion method. The diameter of the inhibition zone indicates the effectiveness of GO/Alg to prevent these bacteria from growing while displaying the nanocomposite as a good material for antibacterial-related application. The best antibacterial properties is a sample of 5.0:2.5ml GO/Alg which shows the highest inhibition zone 0.85cm toward Escherichia coli and 1.03cm toward B. subtilis. These findings demonstrate a simple technique to synthesize GO/Alg nanocomposite for antibacterial purposes.

Keywords: Silver Complex, Hummer’s Method, Inhibition Zone